Phosphatidylinositol phosphodiesterase in higher plants

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Phosphatidylinositol phosphodiesterase in higher plants.

1. The lower regions of the stem of celery (Apium graveolens L.) contain a soluble enzyme that hydrolyses phosphatidylinositol. 2. The lipoidal product of hydrolysis is diacylglycerol, and the water-soluble products are 1:2-cyclic phosphoinositol and phosphoinositol in the approximate proportions of 60% and 40% respectively: this indicates that a phosphodiesterase (phospholipase C-like) activit...

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Phosphatidylinositol 4,5-bisphosphate phosphodiesterase in higher plants.

A phospholipase C which hydrolyses phosphatidylinositol 4,5-bisphosphate to release inositol trisphosphate was detected in a sedimentable fraction from celery and from some other higher plants. The particulate enzyme also hydrolyses phosphatidylinositol, whereas the soluble phosphatidylinositol phosphodiesterase described previously [Irvine, Letcher & Dawson (1980) Biochem. J. 192, 279-283] act...

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Association of phosphatidylinositol 3-kinase with nuclear transcription sites in higher plants.

The kinases responsible for phosphorylation of inositol-containing lipids are essential for many aspects of normal eukaryotic cell function. Genetic and biochemical studies have established that the phosphatidylinositol (PtdIns) 3-kinase encoded by the yeast VPS34 gene is essential for the efficient sorting and delivery of proteins to the vacuole; the kinase encoded by the human VPS34 homolog h...

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Phosphatidylinositol phosphodiesterase in isolated plasma membranes of rodent skeletal muscle

Phosphoinositide metabolism follows cell activation in a variety of excitable tissues (for a recent review, see Hawthorne, 1984). Previous work (Harborne et al., 1978, 1983, 1984) has shown that purified phospholipase C preparations or muscle fractions which exhibited soluble PtdIns phosphodiesterase activity increased the acetylcholine-sensitivity of intact skeletal muscles of the rat and incr...

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Cytokinesis in Higher Plants

structure, the phragmoplast proper (Figure 1B). This *Department of Molecular, Cellular, structure subsequently shortens in length and expands and Developmental Biology in girth until it finally reaches the side wall (Figure 1C). University of Colorado During phragmoplast expansion there appears to be a Boulder, Colorado 80309–0347 shift in the organization of the MTs; whereas the initial † Dep...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1980

ISSN: 0264-6021

DOI: 10.1042/bj1920279